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monoclonal antibodies mouse anti-chicken cd3+ (fluorescein isothiocyanate, fitc staining)  (SouthernBiotech)


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    SouthernBiotech monoclonal antibodies mouse anti-chicken cd3+ (fluorescein isothiocyanate, fitc staining)
    Monoclonal Antibodies Mouse Anti Chicken Cd3+ (Fluorescein Isothiocyanate, Fitc Staining), supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+chicken+cd3+fitc/fitc+conjugated+mouse+anti+chicken+cd4/10__2478_slash_aspr___2023___0039-71-7-19
    Average 90 stars, based on 1 article reviews
    monoclonal antibodies mouse anti-chicken cd3+ (fluorescein isothiocyanate, fitc staining) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Humoral, cellular immunity and efficacy of bioreactor propagated and inactivated Fowl adenovirus 8b adjuvanted with Montanide 71VG in broiler chickens
    Article Snippet: .. After being aliquoted to a Falcon tube (FALCON-Corning, NC, USA), the cells corresponding to 1×10 6 /ml from each sample were stained with mouse anti-chicken CD3-FITC, mouse anti-chicken CD4-APC, and mouse anti-chicken CD8α-PE antibodies (SouthernBiotech, Birmingham, AL, USA). .. After that, the cells were suspended in 500 μl of PBS for CD3+, CD4+, and CD8+ phenotyping using 10,000 live cells on a BD FACS Calibur flow cytometer (BD Biotec, San Diego, CA, USA).

    Article Title: Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants.
    Article Snippet: Journal Pre-proof Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants Pengfei Gu, Adelijiang Wusiman, Yue Zhang, Gaofeng Cai, Shuwen Xu, Shaowu Zhu, Zhenguang Liu, Yuanliang Hu, Jiaguo Liu, Deyun Wang PII: S0378-1135(20)31032-4 DOI: https://doi.org/10.1016/j.vetmic.2020.108894 Reference: VETMIC 108894 To appear in: Veterinary Microbiology Received Date: 19 August 2020 Accepted Date: 11 October 2020 Please cite this article as: Gu P, Wusiman A, Zhang Y, Cai G, Xu S, Zhu S, Liu Z, Hu Y, Liu J, Wang D, Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants, Veterinary Microbiology (2020), doi: https://doi.org/10.1016/j.vetmic.2020.108894 This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Efficacy, humoral, and cell-mediated immune response of inactivated fowl adenovirus 8b propagated in chicken embryo liver cells using bioreactor in broiler chickens
    Article Snippet: The supernatant was discarded, and the cells were suspended in 1 mL PBS and mixed by gentle rocking, counted, and cells equivalent to 1 × 10 6 /mL from each sample were transferred to falcon tubes (FALCON-Corning) for staining. .. Mouse Anti-Chicken CD3-FITC [ ], Mouse Anti-Chicken CD4-APC [ ], and Mouse Anti-Chicken CD8α-PE [ ] antibodies (SouthernBiotech, Birmingham, AL, USA) reconstituted according to the manufacturer’s recommendations were used for staining the prepared cells. ..

    Article Title: Eimeria maxima Rhomboid-like Protein 5 Provided Partial Protection against Homologous Challenge in Forms of Recombinant Protein and DNA Plasmid in Chickens
    Article Snippet: .. An amount of 100 μL of counted lymphocytes were taken from each group and were placed into 2 mL centrifuge tubes; lymphocytes from all of the groups were double stained with mouse anti-chicken CD3-FITC (Southernbiotech, Birmingham, AL, USA) and mouse anti-chicken CD4-PE or mouse anti-chicken CD8-PE by incubating at 4 °C for 45 min under dark conditions; in addition, lymphocytes from the PBS control group were treated with blank or single stain for template adjustment. .. The sample test was run using a FACScan flow cytometer (BD Biosciences).

    Article Title: Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells
    Article Snippet: After dissociation, cells were treated with ACK lysis buffer (A10492, Gibco, Grand Island, NY, USA) for 5 min to remove red blood cells. .. Cells (1 × 10 6 ) were stained for 40 min on ice using the following antibodies: mouse anti-chicken Bu-1 FITC (8395-02), mouse anti-chicken IgM BIOT (8310-08), mouse anti-chicken CD45 SPRD (8270-13), mouse anti-chicken CD3 FITC (8200-02), mouse anti-chicken CD4 Alexa Fluor 647 (8201-31), mouse anti-chicken CD8α BIOT (8405-08), mouse IgG1-FITC (0102-02), mouse IgM-SPRD (0101-13), mouse IgG2b-BIOT (0104-08) and mouse IgG1-Alexa Fluor 647 (0102-31) were purchased from Southern Biotech (Birmingham, AL, USA). .. After washed with PBS, BIOT cells were incubated with Bv421 conjugated secondary antibodies (BD sciences, 563259) for 20 min on ice.

    Activation Assay:

    Article Title: Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants.
    Article Snippet: Journal Pre-proof Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants Pengfei Gu, Adelijiang Wusiman, Yue Zhang, Gaofeng Cai, Shuwen Xu, Shaowu Zhu, Zhenguang Liu, Yuanliang Hu, Jiaguo Liu, Deyun Wang PII: S0378-1135(20)31032-4 DOI: https://doi.org/10.1016/j.vetmic.2020.108894 Reference: VETMIC 108894 To appear in: Veterinary Microbiology Received Date: 19 August 2020 Accepted Date: 11 October 2020 Please cite this article as: Gu P, Wusiman A, Zhang Y, Cai G, Xu S, Zhu S, Liu Z, Hu Y, Liu J, Wang D, Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant for H9N2 vaccine to improve immune responses in chickens compared to Alum and oil-based adjuvants, Veterinary Microbiology (2020), doi: https://doi.org/10.1016/j.vetmic.2020.108894 This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Control:

    Article Title: Eimeria maxima Rhomboid-like Protein 5 Provided Partial Protection against Homologous Challenge in Forms of Recombinant Protein and DNA Plasmid in Chickens
    Article Snippet: .. An amount of 100 μL of counted lymphocytes were taken from each group and were placed into 2 mL centrifuge tubes; lymphocytes from all of the groups were double stained with mouse anti-chicken CD3-FITC (Southernbiotech, Birmingham, AL, USA) and mouse anti-chicken CD4-PE or mouse anti-chicken CD8-PE by incubating at 4 °C for 45 min under dark conditions; in addition, lymphocytes from the PBS control group were treated with blank or single stain for template adjustment. .. The sample test was run using a FACScan flow cytometer (BD Biosciences).



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    SouthernBiotech cd3 mouse anti chicken fitc antibody
    Figure 4. The proportion of the T cell subpopulation in chickens immunized with PBS, pET-32a tag protein and rEmEF2 was determined by flow cytometry 7 days after the first and second immu- nization. (A) Detection of <t>CD3+CD4+</t> T lymphocytes in immunized chickens 7 days after the first immunization. (B) Detection of <t>CD3+CD4+</t> T lymphocytes in immunized chickens 7 days after the second immunization. (C) Detection of <t>CD3+CD8+</t> T lymphocytes in immunized chickens 7 days after the first immunization. (D) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the second immunization. 1: PBS control group. 2: pET-32a tag protein control group. 3: rEmEF2 group.
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    Image Search Results


    Figure 4. The proportion of the T cell subpopulation in chickens immunized with PBS, pET-32a tag protein and rEmEF2 was determined by flow cytometry 7 days after the first and second immu- nization. (A) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the first immunization. (B) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the second immunization. (C) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the first immunization. (D) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the second immunization. 1: PBS control group. 2: pET-32a tag protein control group. 3: rEmEF2 group.

    Journal: Vaccines

    Article Title: Protective Efficacy Induced by the Common Eimeria Antigen Elongation Factor 2 against Challenge with Three Eimeria Species in Chickens.

    doi: 10.3390/vaccines12010018

    Figure Lengend Snippet: Figure 4. The proportion of the T cell subpopulation in chickens immunized with PBS, pET-32a tag protein and rEmEF2 was determined by flow cytometry 7 days after the first and second immu- nization. (A) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the first immunization. (B) Detection of CD3+CD4+ T lymphocytes in immunized chickens 7 days after the second immunization. (C) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the first immunization. (D) Detection of CD3+CD8+ T lymphocytes in immunized chickens 7 days after the second immunization. 1: PBS control group. 2: pET-32a tag protein control group. 3: rEmEF2 group.

    Article Snippet: Whereafter, CD3 mouse anti-chicken FITC antibody (Southern Biotechnology Associates, Birmingham, AL, USA), CD4 mouse anti-chicken PE antibody (Southern Biotechnology Associates, Birmingham, AL, USA) and CD8 mouse anti-chicken PE antibody (Southern Biotechnology Associates, Birmingham, AL, USA) were used to detect the T cell subpopulations with a FACS Calibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

    Techniques: Flow Cytometry, Control